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This paper conducts thermal shock case studies for three typical thermal shock specimens that are important in thermal shock tests: a ceramic layer under a hot shock, a ceramic layer under a cold shock and a ceramic coating under a cold shock.
A general mechanism, identified in E. coli (but not in C. acetobutylicum yet) for the translation of leaderless transcripts, involves the initiation factors 2 (IF-2) and 3 (IF-3) [ 34], where a higher IF-2/IF-3 ratio would favor the transcription of leaderless transcripts, such as has been observed under a cold shock in E. coli [ 34].
To test whether Brn-2 directly interacts with mutant Nhtt in vitro, Brn-2 cDNA was cloned into pCold TF vector; thereby Brn-2 was expressed as a fusion protein with a His tag and a trigger factor (TF) under a cold shock promoter in E. coli, after which His-TF-Brn-2 protein was then purified by nickel column (Supplementary Material, Fig. S8A and C).
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In order to study the biochemical properties of the β-galactosidase enzyme, the bga gene was PCR amplified, cloned, and expressed in the genetically tractable haloarchaeon Halobacterium sp. NRC-1 under the control of a cold shock protein (cspD2) gene promoter.
The gene within clone MrN33-18, encoding a cold shock protein with nucleic acid binding activity, was suppressed under constant cold treatment (GT4, GT10) but induced after 1 h and 4 h (T4, T5) exposure to 4 °C (Fig. 4, group K; Additional files 5 and 6).
Cold shock proteins (CSP) are amongst the most conserved proteins in evolution, sharing a cold shock domain (CSD) from pro- to eukaryotes [ 1].
ScoF, a cold shock protein of S. coelicolor, was used as the internal standard (Fig. 3).
Never pour massage oil straight onto the person's body; it will cause a cold shock.
Thus, a clone of P. pastoris was obtained, which undergoes autolysis after a cold-shock.
We have opted to control the expression of our recombinant proteins by a cold-shock inducible promoter, the E. coli cold-shock protein A (cspA) promoter [ 9, 10].
This protein is a cold-shock induced ribosome binding protein that inhibits translation [ 32].
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