As Grover points out, terms such as "vulnerability" and "abuse of power" remain undefined within the Protocol [ 5].
Although results for primary outcome measures are key determinants of an intervention's benefits or harms profile, we found that the analysis method for primary outcome measures was often undefined in the protocol or altered in the publication.
There is a lack of studies analysing the population of patients with poor-grade SAH, which indeed represent a special cohort of patients, particularly due to the different clinical course with extremely high mortality and morbidity rates as well as the undefined clear treatment protocol.
The check list, accessible at any time, reports any missing annotation, in particular required protocols currently undefined in the project.
We conclude that BMP treatment in the context of this undefined directed mesoderm differentiation protocol strongly interferes with the normal differentiation process in ways that are difficult to interpret, but that clearly extend beyond a simple suppression of EMT.
Additionally T-cell assays are not only frequently conducted with undefined extracts but with different protocols including the addition of cytokines such as IL-2 and IL-7 that can bypass control points.
Others preferred to let protocols remain rather specific to discourage undefined future research.
Our work presents, therefore, for the first time a protocol devoid of serum and other undefined conditions to obtain megakaryocytes from hESCs and hiPCSs capable of shedding platelets in vitro.
However protocols and methods using Me2SO are often undefined, with ambiguous terms such as "work quickly".
There are some issues with these the current differentiation protocols, including spontaneous differentiation, low yield, the presence of undefined and xenogenetic compounds, necessity of cell sorting for specific cell lineages, considerable enzymatic stress during repeated culture (Haque et al., 2011) and cellular heterogeneity in the culture (Nagaoka et al., 2008).
However, generating fully functional cells from hESCs and achieving this goal using clinically-compatible conditions remain major challenges owing to the presence of undefined serum and cellular components in standard culture media and differentiation protocols.
