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All hybridisation bands observed for BlpI digested samples in both the tcsL and parB blots (Fig. 3) were also seen in the uncut samples (data not shown), confirming that the bands shown in Fig. 3 represent linearised versions of the pCS1-type plasmids.
"It is direct and uncut information," he said.
For comparison and to evaluate the impact of removing ambiguous parts of the alignment on molecular clock analyses, we repeated the analysis with the raw (i.e. uncut) alignment of our data (again using the concatenated four-marker dataset in five partitions).
Empirical grinding data is also presented against a measure of uncut chip thickness.
Alex Smith, a spokesperson for UK Uncut, a co-organiser of the protests, described the latest data as unsurprising.
Expression of chd4a mRNA is specific for regenerating fins, as it was not detected in uncut fin folds at the same developmental stage (3 days post-fertilization) (data not shown).
Consequently, we believe that all of the uncut stands were similar enough with the cut ones to be included in the data.
The interview ran uncut.
An uncut gem.
UK Uncut scare you.
Pure Bloody Sam, uncut.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com