Exact(1)
The qPCR reaction included 5 min at 95 °C to activate the polymerase and 50 PCR cycles (uncoupling step at 95 °C for 20 s followed by annealing step at 59 °C for 20 s and elongation step at 72 °C for 30 s), followed by a melting temperature analysis to test for any nonspecific amplification.
Similar(59)
In brown adipose tissue, regulated proton channels called uncoupling proteins can uncouple respiration from ATP synthesis.
Uncoupling protein 2 (uncouplesouples respiration from oxidative phosphorylation.
The modest net effect of cetuximab cycling on IC50 can be eliminated by reducing receptor dimerization and uncoupling rates because cetuximab binding and dissociation steps are no longer rate limiting in the competitive processes described above that allow cetuximab cycling to influence IC50.
Third, translocation of p53 to mitochondria, an important step in the intrinsic apoptotic pathway, is blocked by the uncoupling action of FCCP in JB6 skin cells, whereas UCP2 knockdown promotes p53 translocation (Wang et al, 2010).
The Goop founder and mother of two stepped in it back in March, right before her "conscious uncoupling" announcement, when she barely stopped herself from saying she had it worse than most. .
Green uncoupler development might be another research direction for uncoupling metabolism in MBRs.
Moreover, if EcoR124I occasionally makes short pauses during translocation (see discussion about uncoupling below) or exhibits a slight sequence dependence of its translocation rate, then again the step size would be somewhat overestimated.
THE UNCOUPLING, by Meg Wolitzer.
What is it that causes uncoupling?
Conscious uncoupling – it sounds like something she made up.
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