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What will validation validate?
Those unconfirmed regulators may be suggestive FNs of validation screens, although they could also be missed by the validation screens for a multitude of reasons unrelated to the FN/FP rate (see the discussion).
In fact, while all confirmed snoRNA candidates have large number of sequence reads from late embryo 12-188 h) and S2 cells (Additional file 1, Fig. S6A, B) on which the experimental validations were performed (see Methods), the two unconfirmed box C/D snoRNA candidates have the highest number of sequence reads from early embryo (Additional file 1, Fig. S6B).
A previous validation study found that these cases have a higher proportion of unconfirmed cases [ 18].
One possibility for the relatively low confirmation rate of the data from the AD-C library is that the increases in the expression of unconfirmed transcripts were small and, therefore, might also be difficult to validate by qRT-PCR, although this wasn't evident in the qRT-PCR validation of down regulated genes from the C-AD library.
Using unconfirmed SNPs as discovered in this study the percent of working GoldenGate assays should be the product of the validation rate by the conversion rate of confirmed SNPs.
This, however, remains unconfirmed.
But it is unconfirmed.
His claim is unconfirmed.
Rumors flew, unconfirmed.
Specs: Unconfirmed as yet.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com