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Third, the outbreak strain was assigned to an uncommon sequence type (ST175), which we showed to be highly pathogenic even among non-cystic fibrosis patients.
Approximately half of the isolates belonged to the otherwise uncommon sequence type 677 clonal complex.
Another advantage of the SNP identification from EST sequences is its ability to identify the uncommon sequence variants [ 41].
All these features are also present in the inferred Andalucia tmRNA (fig. 4); however, compared with mitochondrial tmRNAs of the other jakobids, Andalucia's is notable for its condensed secondary structure and shortened T-loop with an uncommon sequence.
The monomorphic SNP rate was highly related to the number of samples tested, since the uncommon sequence variants possess very low minor allele frequency, which required a large number of samples.
We found that nearly half of the isolates belonged to the otherwise uncommon sequence type (ST) 677 clonal complex (CC) (ST-677 CC) 17. Isolates belonging to this particular CC were more resistant to NHS than the other isolates, but otherwise serum resistance was not a prerequisite for bacteraemic infection 17.
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The SNPs derived from contigs with four sequences with only one minor allele sequence had the highest monomorphic rate, and the SNPs derived from more than 10 sequences contigs had the lowest monomorphic rate, suggesting that most of the monomorphic SNPs could have also derived from false SNPs, not uncommon sequences variants.
Interestingly, in two cases the blood samples had an uncommon mtDNA sequence that was reversed by mutation to a frequent variant in tumours: m.16248T>C (1865/2) and m.16292T>C (1801/64).
Lower quality sequences are not uncommon with simple sequence repeats that are large in length as these regions are difficult to sequence through.
The relatively high proportion of reads assigned to introns is not uncommon when the sequencing library preparation includes random priming of the mRNA [ 26].
Afterwards, the starting sequences and putative orthologs were pooled and sequences with uncommon PTS1 tripeptides, that is tripeptides which occurred less than three times, were removed from the set.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com