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Recent findings suggest that excess H2B that is not incorporated into core histones is highly toxic for the cell and is rapidly eliminated by proteasome dependent degradation resulting in virtually unchanged steady state levels of the proteins.
Indeed, it was recently shown that H2B that is not incorporated into core histones is highly toxic for the cell and is rapidly eliminated by proteasome dependent degradation resulting in virtually unchanged steady state levels of the proteins [47].
Unchanged steady state mRNA levels are defined as within the cut-off [1 ≥ (log2mRNAInfected- log2mRNAUninfected) ≥ −1].
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SILAC phosphoproteomic analyses and Western blotting revealed an increase of phosphorylation of stathmin at Ser38 and the unchanged steady-state stathmin in U266 cells upon proteasome inhibition (Fig. 2).
The left-shift of steady-state inactivation was accompanied either by an unchanged steady-state activation (P1298L, R1632H) or by a left-shift of steady-state activation (−15.0 mV for L212P, −6.8 mV for DelF1617; Table 1, Fig. 2B).
In this scenario, loss of NMD factors results in reduced transcription of these NMD targets, compensating for the concurrent increase in mRNA stability, which results in unchanged steady-state expression levels.
Drawing on positive deviance or assets model techniques we compared the experience of districts where diphtheria tetanus pertussis (DTP3)/pentavalent3 (Penta3) coverage improved with districts where DTP3/Penta3 coverage remained unchanged (or steady) over the same period, focusing on basic readiness to deliver immunization services and drivers of coverage improvement.
The slope factor of the steady-state activation curve remained unchanged, whereas the steady-state inactivation curve flattened significantly.
The Labor Department said in a report Thursday that the number of new applications filed for unemployment benefits was unchanged last week, holding steady at 384,000.
After ∼100 days of simulation, the system remains virtually unchanged, characterizing a steady state.
Instead, the inland site had a more stable microbial community (F : B was unchanged) and a steady use of nutrients over the season followed by a second peak of microbial activity at senescence.
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