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In addition, the reduction in Aβ42 did not likely arise from increased degradation, since levels of the two major Aβ-degrading activities, neprilysin (NEP [35], and insulin-degrading enzyme (IDE)[38] were unchanged in brains of control- and rapamycin-fed PDAPP mice as well (Fig. 2f, j k).
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For LC3, we detected unchanged immunoreactivity in brains of anle138b-treated or untreated PS19 mice (Suppl. Fig. 6b).
The Mrp4 protein amount was unchanged in brain capillaries of LPS-treated mice compared with saline-treated mice, while the protein amounts of organic anion transporter 3 (Oat3/Slc22a8) and organic anion transporting polypeptide 1a4 (Oatp1a4/Slco1a4) were decreased by 26%and39%9%, respectively.
In addition, cyclophilin D levels were unchanged in brain cortex and liver of 8-month-old mice (Supplementary Fig. 2B and C), suggesting that cyclophilin D expression induction is target-organ specific and precedes axonal degeneration.
Now, further data suggest that, at least during the second passage in sheep, the biochemical properties (glycoform pattern in brain) of the BSE agent are unchanged (35, 36 ).
In contrast, expression levels of another autophagy protein, ATG5 were unchanged in AD brains, indicating that only portions of the autophagy pathway are de-regulated in AD (Fig. 7A&E).
Occludin immunoreactivity and expression remained essentially unchanged in the brain capillaries of animals in all groups.
Ceramide levels were unchanged in the brain and kidney of B−/− mice.
Somatic/nuclear α-synuclein localization was unchanged in different brain areas (Figure 1D and 5).
However, the glutathione levels remained unchanged in both brain structures.
Brain weight remained essentially unchanged in neonates of all four genotypes, such that it was enlarged in proportion to body weight in Dlk1 +/p and proportionately small in Grb10 m/+ neonates.
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