Consistent with the data obtained from the gene expression analysis, the SAS/FR2 cells clearly expressed higher levels of TS, TP, and cIAP2, with unchanged expression levels of cIAP1 and XIAP compared with the SAS cells.
This could be due to the unchanged expression levels of Ta PHT1.2, Ta PHT1.1 & 1.9 which are still expressed in high Pi environments, or the presence of Ta PHT1.6 which is expressed in roots independently of environmental Pi concentration [ 24].
However, in mouse cells, due to the absence of distal pA site, in cis overexpression of KAT5 cannot trigger APA, and result in the unchanged expression of RNASEH2C.
Unchanged expression of CXCR3A + B by NLGP with downregulation of the CXCR3B indirectly suggests the upregulation of the CXCR3A, responsible for cellular migration.
Western blot analysis showed unchanged expression of sarco endo)plasmic reticulum Ca2+-ATPase (SERCA2a) and phospholamban, associated with upregulated Na+ Ca2+ exchanger levels following acute methamphetamine exposure.
Overexpression of OsMYB4P led to greater expression of Pi transporter-family proteins OsPT1, OsPT2, OsPT4, OsPT7, and OsPT8 in shoots, and to decreased or unchanged expression of these proteins in roots, with the exception of OsPT8.
This invariant set method does not rely on the assumption that 'housekeeping genes' have unchanged expression.
Identification of invariant proteins post hoc guarantees normalization to data with unchanged expression.
Of these, 203 spots were shared (unchanged expression) between the two protein profiles.
Unchanged expression of protein spot was considered if protein intensity was less than two-fold (p>0.05).
Deletion of agr resulted in unchanged expression of saeR expression compared with strain 923 at 3 hours, but a modest decrease at 6 hours (2 fold, p<0.05).
