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Considering the possible involvement of C2A in plant growth and development and its function in response to biotic or abiotic stress [ 12], its unchanged content in leaves would imply a smaller impact on the transgenic plants.
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In CF fibroblasts and platelets, an increased incorporation of choline into membranes has been observed, in parallel to an unchanged PC content, suggesting an increased PC turnover in these cells [46].
Ferrochelatase, another key enzyme in heme biosynthesis, was analysed by Western blotting and showed an unchanged enzyme content during B16 differentiation induced by HMBA or butyrate.
Despite the unchanged sulphur content in mature seeds, we cannot exclude that the composition of sulphur or the amount of sulphate and other sulphur metabolites transported to developing seeds could be different between the wild-type and the sultr4;1 mutant.
Both mutants showed a decrease in eEPSC amplitude, but although this was attributable to defective transmitter release at UNC-51 mutant synapses, no such presynaptic defect was observed in RSK mutants (unchanged quantal content; Fig. 6C).
In tubers of the two AS-h lines with unchanged carotenoid content ("non-expressor" lines), as well as in leaves of all lines, the silencing transcript was below the levels of detection of the Real Time RT-PCR assay.
We found a remarkably high level of sulphate constrasting with an unchanged sulphur content in mature seeds of a sultr4;1 T-DNA mutant, which for the first time implies a function for this transporter in developing seeds.
The decrease in HMGCR mRNA levels was specific, not observed when neurons were infected by a control adenovirus encoding β-galactosidase (Fig 1C), occurred as soon as APP was expressed, and did not result from transient cholesterol overload, as measured by unchanged cholesterol content over time (Supporting Information Fig 2).
However, L-NAME was able to constrain neither complete adipocyte differentiation nor mitochondrial biogenesis (Fig. 1E ), as demonstrated by unchanged triglyceride content (Fig. 1D ) and unaltered levels of PGC-1α and TFAM protein (Fig. 1B ) as well as enhancement of mitochondrial mass (Fig. 1E ).
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