Low quality sequences, including short sequences (less than 100 bp) and those with a high percentage of nucleotide A (adenosine) or N (uncertain read) were considered uninformative and were eliminated from further analysis.
As the transcript of origin is uncertain for reads aligning to shared subsequence, estimation of transcript expression levels has to be completed in a probabilistic manner.
Compared to other risky activities I've engaged in that involve danger and uncertain outcomes (read: chemo), dating isn't too scary, but it is complicated.
Because TEs were called with short-read data, we were able to ascertain the presence or absence of a TE with high confidence; however, identifying the TE family for an individual insertion is much more uncertain with short reads and thus this information was not considered in these analyses.
This may be caused by the introduction of new alternative splicing, repeats and other uncertain factors when pooling reads from different samples.
In 1948, with an Arab invasion imminent, and episodes of ethnic cleansing on both sides portending an uncertain future, David Ben-Gurion read Israel's Declaration of Independence — evoking "the prophets of Israel" and promising "complete equality of social and political rights to all its inhabitants irrespective of religion, race or sex … religion, conscience, language, education, and culture".
Now, when a missing component of an uncertain edge is read while reloading edges from disk, is incremented.
A block of text is titled "Variant of uncertain significance (VUS)." It reads, in part: "I may learn that a VUS was identified by this test.
First, the reads containing adapter sequences or including more than 10% uncertain nucleotides (Ns) were excluded.
At this early stage of Pt genome analysis it is uncertain how much coverage has been obtained with the 454-genomic reads.
