Sequencing uncertainty is reflected in low expression values so genes with uncertain reads are likely to not meet the threshold.
Compared to other risky activities I've engaged in that involve danger and uncertain outcomes (read: chemo), dating isn't too scary, but it is complicated.
As the transcript of origin is uncertain for reads aligning to shared subsequence, estimation of transcript expression levels has to be completed in a probabilistic manner.
In 1948, with an Arab invasion imminent, and episodes of ethnic cleansing on both sides portending an uncertain future, David Ben-Gurion read Israel's Declaration of Independence — evoking "the prophets of Israel" and promising "complete equality of social and political rights to all its inhabitants irrespective of religion, race or sex … religion, conscience, language, education, and culture".
Now, when a missing component of an uncertain edge is read while reloading edges from disk, is incremented.
Because TEs were called with short-read data, we were able to ascertain the presence or absence of a TE with high confidence; however, identifying the TE family for an individual insertion is much more uncertain with short reads and thus this information was not considered in these analyses.
But it would be a mistake to read "An Uncertain Glory" as a screed against liberalization.
In no uncertain terms, the notes read: "What she did for her first six years is her own business".
I'm uncertain you will ever read these words, and even less certain that if you do, they will sound like anything more than whining and finger-pointing.
If after reading the full study they were still uncertain two other team members read the paper and made a final decision.
