Sentence examples for unbroken cells from inspiring English sources

Cell lysates were then incubated for 10 min at 37 °C and centrifuged at 350 g at room temperature for 5 min to pellet unbroken cells.

Unbroken cells were removed by ultracentrifugation at 100,000 × g for 1 h and supernatant (plus 10 mM imidazole) incubated 2 h with Ni-NTA resin (Qiagen).

Further, nuclear fraction and unbroken cells are pelleted at 800 rpm for 10 min at 4 °C.

The homogenate (whole cell) was centrifuged at 1000 g for 5 min to remove unbroken cells and nuclear debris.

The resulting protein extract was centrifuged at 3000 rpm for 10 min to remove unbroken cells and cell debris.

Nuclei and unbroken cells were pelleted at 700 ×g for 5 min, and the homogenization repeated once.

After breaking the cells by using a French press, the unbroken cells were removed by centrifugation at 14,000 rpm for 20 min.

Further, nuclear fraction and unbroken cells are separated from post nuclear fraction by centrifuging at 800 rpm for 10 min at 4 °C.

Alumina, unbroken cells as well as cell debris were decanted after a low speed centrifugation at 3000 rpm for 10 min and the supernatant (crude lysate) was saved.

The homogenate was centrifuged at 800 g for 15 min to remove unbroken cells.

After sonication (8 sec/gram of cells), unbroken cells and debris was removed by ultracentrifugation.