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An amazing moment of creative synchronicity occurred during that missing summer: at exactly the same time when Schubert composed his cantata, Byron wrote his epic poem Prometheus, Frankenstein was born and the seeds of Percy Shelley's Prometheus Unbound were sown.
Comparisons of counts per minute (for radioactivity) or counts per second (for TRF), either as absolute numbers, percentage of total counts, or ratios of bound to unbound were done using a one way (Kruskal-Wallis test with Dunn's test for multiple comparisons) or two-way ANOVA (Bonferroni post-test for multiple comparisons).
The initial 74 chains from test2012 were screened using (Gunasekaran et al., 2004) and 72 of them with MoRFs that were determined as disordered when unbound were kept.
After 20 min, cells that remained unbound were harvested, together with cells from three PBS washes; this constituted the committed basal cells (CBs) that do not rapidly adhere to collagen I.
Both the bound and unbound were supplemented with 10 µl, 4 µg/ml ethidium bromide and scanned by Typhoon scanner (GE Healthcare) and the signals were quantified by ImageQuant TL software (GE Healthcare).
(B and C ) The indicated amounts of RNA from EMCV-infected FLAG-LGP2-expressing HeLa cells (input), RNA associated with LGP2 or control (ctrl) immunoprecipitations (IP), or RNA remaining after LGP2 or control precipitations (unbound) were tested for the ability to stimulate the IFN-β promoter reporter assay in HEK293 cells (B ) or induce IFNB1 mRNA (normalised to GAPDH) in HeLa cells (C ).
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My novel Frankenstein Unbound was optioned by Roger Corman.
· Surrealism: Desire Unbound is at Tate Modern, London SE1 (020-780088008), from September 20-January 1 , 2002
("Zuckerman Unbound is a frictionless read: indeed, it's over before you know it").
Full Circle and Unbound are just two, founded by the veteran publishing stars Liz Calder and John Mitchinson respectively.
(Bound and Unbound is selling, for the most part, work that exists in duplicate form).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com