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Although we are also more interested in the unbound experiments than in the bound, the latter enables us to primitively confirm the advantages of our AEP's improvement at the initial stage.
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For unbound-unbound docking, experiments show that our method significantly outperforms these existing docking approaches.
Interestingly, in these FRET-based ligand receptor binding experiments the unbound fluorescent ligand does not engage in the FRET process, thus only the bound ligand is FRET effective, a fact that allows increasing the signal-to-noise ratio.
Also some recovery of fluorescence is due to the release of bleached E36 from endogenous RNA and subsequent renewed binding of previously unbound E36 during the experiment as described above.
This accords with the results of the in vitro experiments because the unbound plasma concentrations that could be attained in vivo, without causing adverse effects, were far below the Ki values.
Results of long-term aging experiments demonstrate that unbound prions or prions bound to a diverse range of soil surfaces can readily replicate after one year.
b) In order to quantify the diffusion rate of the unbound labeled head, we repeated experiments at much faster imaging speed of 10,000 frames/s.
The small effect on the unbound genes in the RNAi experiments could indicate that they are weakly bound (below what we can detect with ChIP-seq) or that these effects are secondary gene network effects.
The basic tenets he outlined included the importance of getting the compound to the target, the relevant concentration and length of exposure at the target (washout experiments), and the unbound drug being the active moiety.
Sensor 6 (30 μM) was incubated with pancreatic islet cells for 1 h, which were then washed under a steady flow of biological buffer for the remainder of the experiment to remove unbound sensor.
In several, low stringent wash steps, unbound proteins are removed and the experiments are merged into a single tube.
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CEO of Professional Science Editing for Scientists @ prosciediting.com