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elasticus nanoparticles powder in order to remove unbound components.
After immobilization, the transducers were air-dried for about 30 min and then washed from unbound components in the working buffer for about 20 min.
After immobilization, the transducers were dried in air and washed from unbound components of membranes in buffer for 10 to 15 min.
The unbound components, which were monospecific antibodies against digoxin (anti-digoxin), were collected.
Therefore, unbound components cannot be taken up by either other APCs present in PBMCs or slanDCs.
The unbound components, primarily monospecific antibodies against BSA (anti-BSA), were collected.
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Unbound serum components were removed by a subsequent wash cycle.
Each docking returned the 100 most probable models from unbound monomer components.
The following extraction protocol was used: the whole supernatant was applied to SPE columns and unbound sample components removed by washing with 3 mL bidest.
Unbound serum components are washed off and bound antibodies are detected after incubations with anti-human IgG conjugated to HRP Horse Radish Peroxidasee) and specific enzyme substrate (TMB) as per manufacturer's protocol.
Here, the concentration of tracer in the extravascular space of an imaged region has been partitioned into an unbound, diffusing component C d as well as a component C b that is bound by hypoxia.
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