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In order to further explore the effect of cortisol dynamics on target cell response we used an unbiased strategy of expression gene profiling.
To use an unbiased strategy we therefore used injection into fish embryos.
We thus decided to utilize a comprehensive and unbiased strategy to identify the Achilles heel of IBC cells.
Thus, integration of fine resolution QTL mapping, RNA-Seq transcriptome information and mouse muscle Bayesian Network analysis provides a novel and unbiased strategy for nomination of muscle QTGs.
Although Karp et al. chose a targeted approach to measure expression changes of 107 selected miRNA genes, we applied an unbiased strategy that allowed us to detect an unrestricted set of differentially expressed miRNA genes.
Comparison of the numbers of sequence variants found in individuals with trait levels at opposite ends of the distribution provides a simple, unbiased strategy to detect functionally significant sequence variations.
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Due to these advantages, MeDIP-seq is considered to have great potential for the development of cost-effective and unbiased strategies for whole genome DNA methylation profiling.
These unbiased strategies interrogate both strands of the genome allowing the detection of new transcripts such as non-coding RNAs (ncRNAs) and complex transcriptional architectures (Bertone et al. 2004; Nagalakshmi et al. 2008; Garcia-Martinez et al. 2004; Churchman and Weissman 2011; Pelechano et al. 2013).
Furthermore the rational selection and greater density of coverage in these prioritized loci in the IBC array biases towards detection of disease causing loci, that complements the discovery nature of an unbiased GWAS strategy.
Subsequent integration of these datasets permitted design of an unbiased sorting strategy that separates non-HSCs away from HSCs.
We used an unbiased sequencing strategy to characterize the microRNA (miRNA) gene complement in C. elegans, Pristionchus pacificus, and Strongyloides ratti.
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