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The unbiased number of alleles (based on sample size) was calculated in FSTAT, using a method of rarefaction [ 43].
To obtain unbiased number and size estimates from a sample of axons in a nerve cross section, we followed the concept of systematic random sampling using a systematically positioned unbiased counting frame [ 18].
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The initial matrix of CVs of unbiased random number of 25 × 21, 50 × 21 and 75 × 21 (Fig. 2) was generated, saved and subsequently used for training of LVQ1 architecture prior to classification.
Although selection of patients for the study was unbiased, the number of males in the study was more than females.
For an unbiased copy number estimation, genomic DNA of CBS1483 was spiked with plasmid DNA (pUG6 DNA [ 33]).
If the whole-genome amplification and library construction process was unbiased, the number of reads represented in a single contig should have been directly proportional to the length of the contig.
Whilst the methods Gordon and Pont used are not necessarily unbiased estimators, a number of the methods they tested gave estimates of population means with only a slight bias and often with precision close to that of simple random sampling.
Semi-quantification of CCL2- and CCR2-positive cell numbers was performed by unbiased manually counting the number of positive cells with a clearly visible cell nucleus within the region of interest (ROI).
An Olympus BX51 microscope with motorized stage and StereoInvestigator (Version 6.3 Software, Microbrightfield, Williston, VT) were used to stereologically estimate unbiased total cell numbers, cell migration, lesion volume, volume of spared tissue, serotonergic fiber length, NG2 and GFAP areas, and blood vessel length.
To select mutant mice in an unbiased manner, a random number generator was used to obtain catalogue numbers from the Jackson Laboratories.
Since the likelihood for Q sequences is defined as where υ i of x i = ln(T i ) and n i are unbiased variance and the number of time interval data for the i-th sequence, respectively.
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