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The MS/MSALL analysis allows unbiased acquisition of high resolution TOF MS/MS spectra for all potential lipid species across a selected mass range.
MRI can be employed for the rapid, non-destructive and unbiased acquisition of digital morphological data, and the creation of interactive 3D models accessible both within a publication and via the Web.
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Such a practice would not bring about unbiased copying (acquisition in proportion to observed frequency), but rather frequency independent copying, since all treatments would be equally likely to be adopted, irrespective of their frequency or efficacy.
We recently refined the Tm-assay by using a molecular beacon as an internal control for temperature variations over the heat-block in the thermocycler and an automated analysis program for more precise and unbiased Tm acquisition.
An integration of standard toxicological methods with selected pathway-focused bioassays and unbiased data acquisition strategies (such as gene expression analysis) would be advantageous in building an interaction network model to consider all of the effects, whether they were intended or adverse reactions.
Both unbiased UPLC-MS/MS acquisition (DDA, data dependent acquisition), as well as acquisition with no precursor ion selection (UPLC-MSE) were employed.
Some of the newer techniques that implement unbiased data independent acquisition by mass spectrometry followed by targeted data extraction such as SWATH-MS (S equential W indowed data independent A cquisitionof the T otal H igh-resolution M ass S pectra) [100] offer promise for high throughput precise quantification of large number of proteins.
High-content screening (HCS), which combines automated fluorescence microscopy with quantitative image analysis, allows the acquisition of unbiased multiparametric data at the single cell level.
To record chair data such as seat occupancy, back recline angle, lumbar engagement, and armrest location using an independent, unbiased real time data acquisition system.
Serial analysis of gene expression (SAGE) was used for its unbiased approach to quantitative acquisition of transcripts.
Unbiased large-scale data acquisition strategies give insight into transcriptomic, proteomic, and/or metabolomic alterations, which provide more information on all of the activated molecular processes in a system.
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