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This is because the relatively short reads of 102 bp in our study in combination with the unavailability of a reference sequence are not powerful for the detection of InDels.
Marker-assisted selection (MAS) for agronomic traits such as yield, quality, and tolerance to abiotic and biotic stresses is not widely applied in pea due to unavailability of a reference pea genome and the limited number of molecular markers for tagging of agronomically important genes in pea improvement programs (Jain et al., 2012; Smykal et al., 2012).
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Due to the unavailability of A genome reference sequence, we used the genetic map of tetraploid cotton as the main reference in this report.
This approach is especially advantageous for non-model species, because it is not restricted by the unavailability of a genome reference sequence.
Due to the unavailability of a B. napus reference sequence, we applied the following de novo RAD analysis procedure.
The unavailability of a suitable close reference device solution for comparison excluded several large innovations, such as resurfacing total hip replacements and unicompartimental knee arthroplasty.
This sample was also used as an independent quality control sample due to the unavailability of suitable reference material with a comparable matrix.
The 01APPRD tobacco sample was used as an independent quality control sample due to the unavailability of suitable reference material with a comparable matrix.
However, use of this tool as a quality indicator (QI) is hampered by the unavailability of national reference values or benchmarks based on large, multicentric, heterogenous longitudinal patient cohorts.
Moreover, both H.264 and the PVC scheme could not provide the expected RD performance for the uncovered background areas due to the unavailability of the reference areas in the existing approaches.
A major problem in attaining better understanding of mechanical problems at the ankle joint is unavailability of clear reference conditions in terms of either muscle-tendon complex length or muscle force.
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CEO of Professional Science Editing for Scientists @ prosciediting.com