The absolute quantities of each gene transcript cannot be unambiguously compared since the efficiency of the RT step for each primer is not known.
Only sequences that had both the RG(IV T and PGH motifs were used to calculate insertion lengths, as these are then only cases that can be unambiguously compared without actually aligning the paralogs.
PCR-based screening of the DNA pools provides anchoring points quickly and unambiguously compared to the traditional hybridization methods since PCR amplicons with different size can be easily distinguished and simultaneously de-convoluted.
Biological anthropologist Terrence Deacon of UC Berkeley also praises the descriptive work that shows the olfactory bulb was "unambiguously enlarged" compared with the structure in reptiles.
The new method of EST to gene assignment improved accuracy enabling a higher percentage of ESTs to be unambiguously assigned compared to the Huminiecki and Bicknell method [8].
By creating mismatches as permutations of the surface-bound probes, rather than permutations in the target, we are able to unambiguously separate and directly compare the signal from a perfectly matched duplex and several variants.
Some compounds were unambiguously identified by comparing them with the reference compound.
These sequences were unambiguously aligned and compared to the human sequence to determine the ancestral state at each polymorphic position.
The δ and J assignments were unambiguously confirmed by comparing spectra acquired at different operating frequencies.
