They chose this disease because it is caused by a novel gene not found in healthy individuals, and thus provides a clear and unambiguous target for the therapeutic RNA.
Such studies rely on unambiguous target protein recognition by cognate antibodies.
2) In an unambiguous double-related control condition, both primes addressed the same meaning of an unambiguous target (RR) (e.g., lion stripe tiger).
We embarked on this unbiased but laborious approach because the malaria field has yet to identify unambiguous targets of protective antibodies.
An aggressive act occurred when one individual threatened an unambiguous target with at least one of the following behaviors: threat grunts, head bob and ground slaps, lunges, chases, and/or bites.
These included 2 unambiguous single-related trials (R1: lion bread tiger; R2: stripe rest tiger), analogue to the ambiguous conditions Rd and Rs, and 2 novel unrelated conditions, in which none of the primes were related to either an ambiguous (UUa; pillow clock ball) or unambiguous target (UU; bread rest tiger).
Because unambiguous targets of protective Abs have yet to be identified in malaria, another approach involves cloning and expressing antibodies from bulk-sorted atypical and classical MBCs to compare their specificity profiles in an unbiased manner using protein microarrays that represent the entire P. falciparum proteome of over 5400 gene products.
Therefore, M5 lever arm motion was naturally chosen as a paradigm target to illustrate the power of HS-AFM in providing unambiguous direct data of complex molecular processes.
With targeted application of laboratory procedures that enable unambiguous phase determination via physical isolation of alleles from diploid PCR products, relatively little investment of time and effort is needed to overcome the observed biases.
The users of the marmoset microarray have access to the exact probe set sequences as well as the EST sequences, allowing unambiguous determination of which part of the transcript is targeted and which splice variants or isoforms are detected.
