An essential, but sometimes overlooked issue, is the availability of a detailed, concise and unambiguous study protocol with guidelines for delineation of the target volumes[ 32 ].
To create better-defined models of AR signaling in human breast epithelial cells, we took estrogen receptor (ER -α-negative and progER -α-negativetor (PR)-nER -α-negativebreand eprogesteronell lines, both cancereceptor non-cancerous, and engineered them to exPR -negativeus allowing the unambiguous study of AR signaling.
Furthermore, to ensure that the survey questions and response categories were clear and unambiguous, a pilot study was conducted at the behavioral research lab of the London School of Economics with a focus group of (N = 15) members of the general public.
In the present study, unambiguous evidence for such a large genomic deletion within DPYD was obtained in a patient with reduced DPD activity and severe 5FU toxicity.
Detection of both parvovirus B19 and PARV4 sequences was highly reproducible between the 2 tissues analyzed (Table 2), which enabled generally unambiguous categorization of study subjects into infected and uninfected categories.
A power calculation was not undertaken a priori, making both the attainment of statistical significance here less important, and relatedly, clear and unambiguous interpretation of study findings more challenging.
In the current study, unambiguous preference scenarios supported highly certain inferences because of what perceivers knew about their protagonists (i.e., the person), for example, that someone chose a particular item consistently despite having other options.
It is evident from these discrepant observations that unambiguous interpretation of studies with Ucp2-ablated mice is difficult given the confounding effects of genetic background.
For unambiguous identification fragmentation studies were performed by Ultra High Performance Liquid Chromatography (1290 Infinity, Agilent Technologies) coupled with a 6540 UHD Accurate-Mass QTOF LC MS instrument with Agilent Jet Stream Technology as ESI source (Agilent Technologies) as described (Koch et al., 2013).
These results suggest that DOSING may be effective in correcting dosing errors that are due to lack of knowledge or awareness (e.g. of serious adverse effects) and for which the evidence provided is unambiguous, but further studies with larger sample sizes are necessary to confirm these findings.
However, in analogy to the neuropsychological findings, these results do not seem to be unambiguous, since other studies reported correlations between negative symptomatology and anomalies in other brain areas, especially the temporal regions, with the superior temporal gyrus [ 34, 41- 43] and the hippocampus being most often reported [ 44].
