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Nevertheless, irrespective of the selected strategy, efficient collection and preservation of banana diversity highly depend on unambiguous sample identification.
The long nucleotides offer strong hybridization signal, good specificity, unambiguous sample identification, and affordability (Hughes et al. 2000; Kane et al. 2000; Schena et al. 1998).
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The main finding is unambiguous: fixating the sample dots improves the subsequent reproduction of a distance separating two sample dots in both, general accuracy and in precision of responses.
We obtained between 45 and 85 million unambiguous alignments per sample.
Unlike the research and clinical contexts for which this instrument was designed (where both the 'before' and 'now' points will be unambiguous), our purposive sample asked individuals to identify a relevant time frame over which they would be doing the recall, and then to maintain that time frame while completing the questionnaire.
Melting peaks were mostly unambiguous, but certain samples exhibited abnormal peaks due to two rare silent genetic polymorphisms, one covered by the sensor (G645A [Leu215]) and another covered by the anchor probe (G654A [G654A8]).
From the available 590 gDNA samples, 510 samples gave unambiguous digestion result and were considered for subsequent analysis.
Furthermore, while earlier ToF-SIMS analyses of cellulase activity on spruce samples distinguished control and enzyme treated samples by unambiguous enrichment of polysaccharide and lignin peaks, respectively [ 1], in the present study, the PC2 loadings plot described the control samples by both polysaccharide and lignin-descriptive peaks.
An average of 34,278 unambiguous clean tags per sample were calculated for each gene and then normalized to TPM, which linked the tag numbers with gene expression levels.
The isotope distribution fits for S17 peptides are often poor for both the experimental and reference sample, preventing unambiguous assignment of these peptides and necessitating their exclusion from the qMS analysis for some fractions.
Of samples that quantitated with <10 copies (5 μl -1 in a final quantitation assay of all our DNA extracts, 39/41 had previously produced unambiguous sequence data, while samples that did not amplify in this reaction (68 samples) had usually failed to produce sequence data in previous positive PCRs (though 8 had previously produced successful sequences).
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