Parsimony bootstrap values indicating monophyly of Microbotryum group I, for instance, range from 57% under the MAFFT alignment to 91% under the POA alignment, and the group receives no support from alignment-unambiguous regions alone.
For +3s, three ambiguous regions fall between the four unambiguous outcome regions.
We obtained a set of unambiguous enrichment regions at each dpi by merging peak calls across samples.
The liftOver program (Kent et al. 2003) was used to identify singly mapped (unambiguous) orthologous regions in the nonmouse species genomes.
Iodine stain showed unambiguous lightly stained region (Fig. 1b), which suggested that the tumor was covered by normal esophageal mucosa.
Where the similarity between sequences is less than 30%, it can be extremely difficult to align sequences in a certain and unambiguous way; this region of similarity is often described as the 'twilight zone'.
In summary, for the majority (69%) of putative coding transcripts, a respective unambiguous syntenic chromosomal region could be mapped in the human genome assembly indicating a high degree of conservation of transcriptional activity.
Lowering the sequence identity from 85% to 75% and reducing the match lengths to less than 200bp did not increase the number of unambiguous chromosome rearrangement regions (data not shown).
While the link between computations involved in processing complex syntax however conceptualized and left frontotemporal regions seems unambiguous, the processing (and violation) of local grammatical structures activates regions in right temporal lobes in addition to the FOP and L STG/MTG [Friederici et al., 2000a; Friederici et al., 2003; Friederici et al., 2006a; Ni et al., 2000].
Modification of the well-conserved loop separating TM4−5 did not alter Slc11a2 functional expression, providing unambiguous data in a region that had not been previously probed in any Slc11 members.
This apparent drawback turns out to be an advantage as the overlay of on- and off-resonance CARS measurements of the same region provides unambiguous determination of the location of each MPIO within the cell, and intrinsically discriminates between them and micrometer-size lipid structures that may easily be confused in brightfield images of the cells.
