As with OAS1, only good quality, unambiguous resequencing data were used for the haplotype analysis.
We were interested in further study of the two discrepant genotype cases, since both had previously been concordant with HapMap in the 7-reaction-multiplex PCR data set, and both showed high quality, unambiguous SNP Charts in the 50-plex PCR data set.
Except from a forensic point of view, they are unambiguous, and have the quality of pornography.
We've talked about it on this blog before and no doubt the discussion will continue after it passes or is rejected, but it's important at this critical moment that everyone concerned weigh in and make an unambiguous statement regarding the quality of this bill.
In both structures, the ligand electron density allowed definitive placement of the phosphate group and illustrated the general path of the carbon chain, but was of insufficient quality for unambiguous placement of all ligand atoms (Fig. 5a, b).
The resulting map was in good quality for unambiguous sequence assignment with the aid of selenium sites in the anomalous difference Fourier map.
Although the density for the kinked α-helix was not of sufficient quality for unambiguous identification, mass spectrometry and Western blot analyses suggest that a mixture of different proteins with a single transmembrane helix may be present in our sample.
Structure also seems to be related to outcome characteristics, such as clear and unambiguous goals for the quality system, periodical evaluations of the goals, documentation of the results of the evaluations and feedback of the results to the staff.
We used an iterative alignment procedure similar to Imakaev et al. (2012), that is, only the first 20 base pairs of the read were initially mapped then the length of the read was progressively increased until the mapping became unambiguous (with a mapping quality superior to 40).
Alignments were relatively unambiguous after being trimmed for quality and maximum coverage (i.e. ambiguous end regions were removed, and most sequences began and ended at the same point).
