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All 29 loci amplified an unambiguous product of the expected size.
Twenty-four loci amplified an unambiguous product of the expected size.
The GC-MS analysis of recombinant enzyme product showed a much higher product yield compared to the in vitro assay, but the fragmentation pattern of the obtained products still did not allow unambiguous product identification.
Twenty-four loci amplified an unambiguous product of the expected size and these loci were then screened on eight individuals to test for polymorphism using an ABI 3730 genetic analyzer (Applied Biosystems, Foster City, California, USA) and GENEMAPPER version 4.0 software (Applied Biosystems).
Preparative-scale experiments were performed to allow unambiguous product identification by HRMS and H NMR, C NMR, and/or F NMR spectroscopy and thus to ascertain that the L384G-catalyzed amination of 2 b– f, 2 h, and 2 i yields the corresponding amino acid products 1 b– f, 1 h, and 1 i (Scheme 1).
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Sequences of final primer pairs yielding unambiguous products are provided in Table S2.
2, 3, 5b The DNA sequence is chosen in a way that an unambiguous hybridization product is formed.
Out of 125 primer pairs designed, 110 (88%) could generate unambiguous amplification products.
Fourteen out of the 48 primer pairs designed to amplify SSR, produced unambiguous polymorphic products in our test population of 94 isolates.
Twenty-four oft of 49 primer pairs designed to amplify SSR, produced unambiguous polymorphic products in our test population of six isolates.
No further unambiguous PCR products were obtained linking the ends of the contigs.
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