While this exclusion list strategy enhances the information that can be obtained during sample analysis, complete and unambiguous RNA modification mapping still requires the use of multiple RNases to provide appropriate sequence coverage.
While a significant number of the modified digestion products can be mapped back onto a unique genomic tRNA sequence, complete modification mapping and unambiguous sequence assignment requires complementary data from additional RNase digestions.
Although not as sensitive as antibody-based characterization of histone modifications, MS procedures offer a highly quantitative, unambiguous and generally unbiased identification of histone modifications that often cannot be easily controlled for otherwise [ 22, 23].
The fourth filter reduced FindMod false positives by considering whether modifications found by FindMod were unambiguous or ambiguous.
Atom modification primitives are mostly straightforward and unambiguous, such as changing an element label, or altering atom-centred properties such as charge or radical count.
"Many oppose germ-line modification on the grounds that permitting even unambiguous therapeutic intervention could start us down a path towards non-therapeutic genetic enhancement.
Northern blotting of sRNA- Xoo2 revealed two unambiguous bands, suggesting a possible presence of post-transcriptional modification of the sRNA- Xoo2 primary transcript.
The software in different searches may position the modification to different sites when there is insufficient information for unambiguous site determination and neither assignment contradicts the data.
Questions were assessed to be unambiguous and socially apt; no problems were identified and hence, no further modifications were required.
The presence of the poly(A) tail is an unambiguous criterion to determine the EST orientation; however, the nature of the 3' end post-transcriptional modifications in mitochondria varies significantly among organisms.
