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In order to determine whether Wnt/β-catenin signaling induces the fate-change of endodermal cells into hepatoblasts, we chose 26 hpf, a time when the non-hepatic endodermal region can be clearly defined, thereby allowing for the unambiguous labeling of non-hepatic endodermal cells for lineage tracing.
Depending on the mutant phenotype, scoring is not always completely clear, and even when the phenotype is unambiguous, labeling errors can happen.
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The only unambiguous labelling of genes is in terms of the protein they code for".
1. Biological functionality is multi-level 2. Transmission of information is not one-way from the genes 3. DNA is not the sole transmitter of inheritance 4. The theory of biological relativity means there is no privileged level of causality 5. Geneontology ("naming") will fail without higher-level insight; the only unambiguous labelling of genes is by the protein they code for 6.
Key features are the batch-wise exchange of the chemical solutions, the reusability of all major parts and the easy and unambiguous labelling of each individual sample.
In other words, we need to collect a corpus of web pages, define a set of unambiguous category labels, and confirm that observers agree on the ground truth labeling of those web pages' categories.
These include the concept that the molecular components of a pathway be they proteins, protein complexes and genes (or in principle any other cellular component that plays a part in a pathway) are represented as simple shapes containing a unique and unambiguous identifying label.
The main requirement for an identifier is that the label must be unambiguous: the same label must always refer to the same substance, and no other substance may have this label.
If a name is labelled "unambiguous", every occurrence of it will be removed from the text.
By contrast, F MRI provides a more accurate, unambiguous detection of labeled cells (given the lack of background signal).
An identifier is a single unambiguous string or label or name, that references or identifies an entity or object (that can be a publication, a database, a protein, a gene, etc).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com