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We conclude that the incorporation of specific C and F labels within intact peptidoglycan followed by frequency-selected, one-dimensional, solid-state NMR detection has led to unambiguous insights into the complex structure of the short-bridged Gram-positive bacterial cell wall.
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Experimental 3D structures with bound substrates of different chain lengths, which could give unambiguous insight into residues participating in substrate selectivity, are currently unknown.
The set of on/ off resonance CARS images provides unambiguous insight into the presence and three dimensional distribution of single MPIOs, granting access to information that is otherwise impossible to obtain from the brightfield image in Fig. 5a.
Thanks to the fast time responses and continuous analysis of microdialysate streams, this study has allowed an unambiguous insight into the timing of the chemical events that occur during an SD wave, a distinct advantage over the currently used flow injection system.
1. Biological functionality is multi-level 2. Transmission of information is not one-way from the genes 3. DNA is not the sole transmitter of inheritance 4. The theory of biological relativity means there is no privileged level of causality 5. Geneontology ("naming") will fail without higher-level insight; the only unambiguous labelling of genes is by the protein they code for 6.
There is little unambiguous appeal to direct mystical experience or insight.
The used multi-technique approach has allowed the unambiguous identification of the red pigment as red ochre and has provided insight on the provenance of both the pigment and the material used for the realization of the wall paintings.
Unambiguous identification of a natural metastable protein assemblage may require more comprehensive calculations coupled with insight gained from experiments and observations in the field.
This work provides the first unambiguous evidence for the presence of a fourth UCP gene in teleost fish genome and brings new insights into the evolutionary history of the gene family.
To obtain an unambiguous picture of the ancient genetic relationships between the breeds in our dataset and, thus gain insight into the origin of the Merino breed is not straightforward.
Wittgenstein's warnings against premature theorising and overgeneralising, and his insight that many everyday terms pick out families, should mandate caution over expecting a complete and unambiguous specification of what constitutes a necessary, or a sufficient, condition.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com