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There were unambiguous hits for 208 of 231 (90%) gene-associated markers and for 48 of 124 (39%) microsatellite loci.
For each of these methods details about the inputs, search databases, search parameters and detection of unambiguous hits leading to recognition of relationships are described below.
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Two layers are wound in opposite directions, the third is parallel to the incident beam direction, resulting in an unambiguous hit reconstruction and a position resolution better than 1.6 mm for charged particles.
Now, because Shively had both sold her story to the press and seemingly tried to hide the transaction from authorities, her testimony was thrown out -- an unambiguous hit to the prosecution's case.
For each gene, the best unambiguous hit was retained.
Only those primers that gave an unambiguous hit with one contig or singleton or "single locus sequence group" were recorded as candidate SSR.
However, only 1,024 C/C m/ z pairs were identified from all the spectra, leading to unambiguous database hits.
The Pap3 search revealed an unambiguous best hit in Manduca sexta, with Pap2 and a number of hemolymph proteinases as alternatives within the same species, but with considerably less similarity.
It was the only unambiguous BLAST hit to the Rfam with a bit score (27.3) between the biologically significant and minimal significant bit score cut-offs.
If multiple genes produced identical bit scores with a given contig/singleton, ties were broken as follows: i) if exactly one of tied genes gave unambiguous best hit with some other contig(s) or singleton(s), this gene was selected, ii) in the remaining cases ties were broken randomly.
The 1435 cDNA fragments generated 1050 unambiguous murine genomic hits, 19 ambiguous multiple hits, and 104 alignments with mitochondrial DNA.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com