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The alignment was straightforward and required only a few unambiguous gaps in the Mll intron.
Twenty-one unambiguous gaps in the ITS matrix, 51 in the trnS-trnG matrix, and ten in the psbA-trnH matrix were coded as binary characters based on Simmons & Ochoterena [ 69].
However, some fiber-FISH signals showed unambiguous gaps in the junction between telomeric DNA and the CL14/CL34 signals, suggesting the presence of additional DNA sequences at the junctions.
Similar(57)
The alignment of the exon sequence was unambiguous without gaps.
Alignment of the protein coding regions was unambiguous, a few gap positions are only found within a non-coding region between cox3 and trnQ and within the transfer RNA.
Multiple sequence alignments for H3 and COI were unambiguous and contained no gaps.
The introduction of CLE enabled unambiguous identification of epithelial gaps in patients.
For both COI and ITS2 the alignments were unambiguous, the former with no gaps, and the latter with only a few, unambiguously placed gaps.
For both markers, histograms of p-distances comprising the entire dataset showed no clear gap, while every site specific sub-sample presented a gap, seemingly defining an unambiguous limit for intra- and inter- genetic diversity.
Using this method, we closed 15 gaps between segments with unambiguous sequences.
The alignments of H3 and COI sequences did not require insertion of gaps and was therefore unambiguous.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com