The consensus panel ensured the content of each item was relevant, unambiguous, and framed in a culturally sensitive manner.
Of these sequences, 109 ESTs (64%) contained unambiguous open reading frames with an annotated ATG translational start site at their 5' end, and 44 ESTs (26%) did not contain an ATG that aligned with the start sites of corresponding database sequences.
Syntenous blocks of ' Candidatus Phytoplasma australiense' isolates PAa and SLY, as determined by unambiguous orthologus Open Reading Frames (ORFs).
All the open reading frames (ORFs) with unambiguous correspondence in S. cerevisiae, S. paradoxus and S. mikatae (listed by Kellis and collaborators in the web page ftp://ftp-genome.wi.mit.edu/pub/annotation/fungi/comp_yeasts/S1b.ORFs/listing.txt) were selected in a first step.
In such cases, we selected the octanucleotide with closest resemblance to TATA-box (i.e., with fewest nucleotides different from consensus TATA sequence) between two framing regions of unambiguous alignment, and considered it to be the ortholog of the TATA box.
For the uniquely mapped clones, 995 of them were unambiguous fragments of mRNAs according to the open reading frame (ORF) annotations of the National Center for Biotechnology Information (NCBI).
We take the maximum ratio over all time frames to represent the threshold for the unambiguous detection of shallow sources: ~0.18 for the European array and ~0.04 for the USArray.
The three genes used were not length variable and the unambiguous alignment was based on conservation of amino acid reading frame.
Unlike the research and clinical contexts for which this instrument was designed (where both the 'before' and 'now' points will be unambiguous), our purposive sample asked individuals to identify a relevant time frame over which they would be doing the recall, and then to maintain that time frame while completing the questionnaire.
Because the particles move slowly compared to the video frame rate (30 frames per second), frame-to-frame particle identification is unambiguous.
