Unambiguous base compositions were derived from the exact mass measurements of the complementary single-stranded oligonucleotides[26]. Quantitative results are obtained by comparing the peak heights with an internal PCR calibration standard present in every PCR well at 100 molecules[20].
For four of the unknown metabolites, unambiguous elemental compositions were annotated (m/ z 647.3724 at 23.9 and 26.74 min, m/ z 651.5653 and m/ z 787.5031).
The latter-day insistence on unambiguous originality in musical composition — or in literature, for that matter — betrays a small-mindedness about the nature of creativity.
Inter-individual distance within groups was typically less than six body lengths and groups were small (≤16 individuals) so group composition was unambiguous.
Taken together, these data-driven approaches permit unambiguous assignment of elemental composition to all queried MS features in this study.
In this report, we introduce two new technologies that hold promise to further facilitate unambiguous assignment of elemental composition in discovery, GC/MS-based metabolomic analyses.
We demonstrate the unsupervised, consistent acquisition of structurally rich MS/MS spectra for intact ion species from nearly all MS features over multiple analyses, knowledge of the carbon and nitrogen content in all MS and MS/MS peaks, and finally, unambiguous assignment of elemental compositions for all queried features.
FT-IR spectroscopy leads to unambiguous information about the stone composition, both for main substances and trace elements, all essentials to guide therapy [ 6].
The relationship between stop codon frequency and G-content, or GC-content as reported previously [ 14], is one of the most striking and unambiguous patterns in bacterial genome composition.
Although LC-HRMS /MS) aLC-HRMS /MS the generalson of structurenablesed informathen for the measured substances, the unambigenerationidatiof of the elemental composition and detailed chemical structure of unknown metabolinformations one oforhe mosthehallenging tasks in untargeted measuredmicsubstances
