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Q-PCR analysis is often unable to reliably detect small (less than twofold) differences, especially when there is some biological variability.
Thus, the Greer et al. (2002) study was unable to reliably detect up to a 40% change in RAIU at the lowest dose tested.
Whether the apparent target of separase activity, cohesin, localizes to centrosomes remains unclear since we were unable to reliably detect cohesin in the early embryo (data not shown).
However, cytological and histological techniques have limited sensitivity and are unable to reliably detect minimal disease (MD) (Cheung et al, 1997).
Although genotypic testing is more sensitive than phenotypic testing at detecting mutations present as part of a mixture, standard genotypic testing is unable to reliably detect minority drug-resistant variants present at levels below 20% of the circulating virus population.
In any event, the commonly used cadmium hemoglobin assay is unable to reliably detect MT protein in the blood, probably because of abundant sample hemoglobin and interference by other blood components.
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We were unable to culture enough NCS proband (p.Phe296Leufs*26) fibroblasts to reliably detect a WDR73 band by immunoblotting, but would predict reduced abundance of both WDR73 p.Phe296Leufs*26 and p.Arg256Profs*18 based on instability of the recombinant versions of these proteins (Fig. 6F and G).
Because of the absence of suitable antibodies to reliably detect endogenous p73, we were unable to confirm knockdown of p73 at the protein level.
… This enabled the Nsen to reliably detect the stimulus.
However, it is still conventionally challenging to reliably detect boundaries.
It may have had an effect but the study simply did not have the statistical power to reliably detect it.
More suggestions(15)
impossible to reliably detect
unable to conclusively detect
unable to clearly detect
unable to confidently detect
unable to reliably distinguish
unable to reliably ascertain
unable to reliably dissect
unable to reliably allow
unable to reliably obtain
unable to reliably reproduce
unable to reliably measure
unable to reliably quantify
unable to reliably analyze
unable to reliably communicate
unable to reliably prevent
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