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The adoptive transfer of lymphocyte subsets from control (BSA) mice was unable to reconstitute protective immunity in any instance.
But failure would also be expected if the sheer size of the large Stentor-masses rendered them unable to reconstitute a normal basal-apical morphogenetic gradient.
By both noncompetitive and competitive reconstruction assays, we clearly demonstrated that the HSPCs from Rps27l−/− fetal livers were unable to reconstitute sterilized bone marrow.
We have shown that three reconstituted in-frame isoforms, i7ec, d5f and d10f, are inactive, being unable to reconstitute telomerase activity in vitro with either vTR or chTR.
This effect was cell intrinsic as competitive repopulation experiments in Rag2 knockout mice demonstrated that cells lacking PDK1 were unable to reconstitute the pre-B cell population in the bone marrow.
Expression of FKBP-hDyn in the absence of AP20187 did not alter the fraction of monopolar spindles (47.8 ± 2.7%; mean ± SD), indicating that the monomeric version of dynein was unable to reconstitute the function of native dynein.
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In contrast, bone marrow from PDK1fl/fl/Vav-Cre+ve mice was unable to effectively reconstitute either T or B cells in the Rag2 knockout mice (Supplementary Figure 7).
The deletion of gB rendered BoHV-4 unable to be reconstituted and productively replicated.
RSV was unable to activate PRDIII luciferase reporter activity in the cells transfected with empty vector or in those reconstituted with IKKγΔ (Figure 5D).
Pre-HSCs were unable to engraft and to reconstitute the hematopoietic system in lethally irradiated adult mice.
Most importantly, reconstituting α-SNAP deficient cells with a previously reported mutant of α-SNAP, L294A (Barnard et al., 1997), that is unable to activate NSF and promote SNARE complex disassembly, failed to reconstitute transferrin flux through the endocytic pathway but fully restored SOCE.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com