Sentence examples for unable to amplify and from inspiring English sources
As a result, we were unable to amplify and sequence either idiomorph in its entirety.
We were unable to amplify and sequence the UGT1A6 gene in any of the three otariid species we sampled, despite using a variety of PCR primer sets that had worked in all other species, and readily obtaining the UGT1A1 gene sequence in all three species.
We were unable to amplify the F3H and DFR loci because the location of multiple introns with poly-thymine tracts prevented us from generating reliable sequences.
Mad had been excluded from preliminary analyses due to a lack of 454 raw reads for the gene, and we were unable to amplify DALR from other races and species.
A homozygous exon-spanning deletion was suspected when we were unable to amplify the exons 19, 20 and 21 by PCR and consequently we did not obtain any read for these amplicons by NGS.
Although we were able to detect PCR fragments derived from DNA upstream of the P-element insertion site in both wild-type and mutant embryos, we were unable to amplify fragments downstream of it (primers 10 and 3; see Materials and Methods).
We succeeded to generate full-length cDNAs for three additional genes, At1g17480, At1g18840 and At4g23060, but were unable to amplify cDNAs for At1g51960, At2g02790, At3g22190 and At3g49380.
We were unable to amplify speL from any isolate and therefore were unable to generate a positive control for speL.
For several individuals we were unable to amplify the ~5.5 Kb fragment, and instead used additional allele-specific primers placed ~500 bp 3' of the CDS to amplify this region in two parts.
In the course of this study, we encountered CO2-sensitive individuals of other species of flies from which we were unable to amplify virus using our primers, and these may harbour other sigma-like viruses.
We considered this most likely a laboratory contaminant, since we did not identify any other reads to filoviruses and were unable to amplify additional filovirus sequence from BC-22.
