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Histological and ultrastructural sections of simple non-induced spheroids confirmed the fusiform aspect of cells at the periphery (Fig. 1E, H).
Histological and ultrastructural sections, however, confirmed extensive disruption of the extracellular collagen matrix and inter-fibrillar proteoglycan associations following cryopreservation.
Differential stainings for histological sections are a further bonus of the latter techniques and it is principally possible to generate digital tomographic data from consecutive histological or ultrastructural sections [ 25].
Semithin sections stained with 1 % methylene blue were used to select suitable areas of ultrastructural sectioning.
For ultrastructural observations, ultrathin sections of 75-nm thickness were cut on an ultramicrotome (Leica EM UC6, Germany) with a diamond knife, and were mounted in copper grids with 300 square mesh.
For ultrastructural analyses, 70-nm thicrossosectionsofs of the nerves were obtained and stained with citrate/uranyl acetate.
Ultrastructural EM analysis of transverse sections of sciatic and vagus nerves showed no significant defects in the myelin structure of JAM-C SC KO fibers or in the "kissing points" of tight junctions on the mesaxons as compared to WT nerve fibers (data not shown).
In addition, pathologic studies allowed us to detect trypomastigotes and amastigotes of Trypanosoma sp. in the SOL of the CNS according to cytological (Giemsa-stained imprints of leptomeninges), histopathological (Hematoxylin-Eosin stained sections of SOL), and ultrastructural criteria.
Semithin and ultrastructural histology of superior to inferior oriented sagittal sections of the retina were performed as previously described (19, 32).
To determine if ultrastructural changes to the glycocalyx accompany regeneration, thin sections of the glycocalyx (preserved by using a fixative mixed with tannic acid and ruthenium red) were observed in the transmission electron microscope.
Ultrathin sections of KMnO4-fixed cells were used for ultrastructural analysis as described [ 56].
