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Electron microscopic analyses have revealed exquisite ultrastructural images of cell structure, but this technique typically requires extensive processing procedures that are labor intensive and time consuming.
In response to cytostatic agents, ultrastructural images of endodermal cells engulfing dying interstitial cells have been captured [57], and very recently molecular evidences have shown the induction of autophagy both during starvation and regeneration [58].
Ultrastructural images of control group present clearly shaped myelinated fibers.
The first report with properly interpreted ultrastructural images of autophagic structures dates back to 1959 by Novikoff [ 6].
These observations were confirmed on ultrastructural images of semi-thin sections (Fig. 2H, supplementary material Fig. S5A).
A similar "bodies like" pathway of transport is also pointed out by the ultrastructural images of Xenopus oocytes containing nuage (Biliński et al. 2004).
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We acknowledge David Carter at the Institute for Integrative Genome Biology and Steven McDaniel at the Center for Advanced Microscopy and Microanalysis in UCR for assisting microscopic image acquisition and ultrastructural image analysis, respectively.
Sample processing, sectioning and examination of the transmission electron microscope were performed in the Ultrastructural Imaging Unit, King's College London.
We acknowledge MR4 and Prof. Leann Tilley (La Trobe University, Bundoora) for the CRT antibodies, Dr. Mike Blackman, Division of Parasitology for rabbit anti-MSP1 serum, the Centre for Ultrastructural Imaging, Guy's Hospital, for EM assistance, Dr. Pauline Bennett for Protein A-gold, and Ian Williams for technical support.
Cells of pyramidal layer reveal a correct ultrastructural image.
Using TCP in animals intoxicated with dioxins limits changes in histopathological and ultrastructural image.
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