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We designed a protocol for reliable ultrastructural identification of proliferating cells in a tissue volume using DNA-incorporated 5-bromo-2-deoxyuridine (BrdU) as a marker.
Co-localization of nuclear apoptotic 3′-DNA fragments within identified hyperlipidemic granulosa cells was coincident with the cytochemical and ultrastructural identification of lipid penetration through the nuclear envelope in db/db mutants.
The co-localization of nuclear apoptotic 3′-DNA fragments within identified hyperlipidemic granulosa cells was coincident with the cytochemical and ultrastructural identification of lipid penetration through the nuclear envelope in db/db mutants.
The ultrastructural identification of MCVyP viral particles in primary MCC tumour cell cytoplasm and nuclei in another patient, suggests active viral replication.
Positive staining with HMFG2 and ultrastructural identification of desmosomes confirmed the epithelial nature of the cell lines.
Kirkham M, Fujita A, Chadda R, Nixon SJ, Kurzchalia TV, et al. (2005) Ultrastructural identification of uncoated caveolin-independent early endocytic vehicles.
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Transmission electron microscopy has allowed the identification of ultrastructural features of the adult S. japonicum, some of which differ from the reported features of other schistosome species.
Ultimately, resolving deep nodes will require the use of multigene alignments incorporating a wide diversity of taxa combined with the identification of robust ultrastructural or molecular synapomorphies for proposed clades.
Technical developments that will allow a more direct comparison with our data include protocols that optimize the time of tissue processing and sampling strategies, such as automated ultrastructural reconstructions, improved ultrastructural stereological quantifications and light microscopy-based identification of putative synapses (Denk and Horstmann 2004; Vanhecke et al. 2007) (see below).
Electron microscopy techniques can be a useful tool in drug studies for the identification of cell surfaces and ultrastructural alterations, as well as specific organelle targets in the parasites.
Ultrastructural analysis of sea urchin eggs allowed the identification of several granules.
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