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Histopathologic and ultrastructural evaluation was assessed at 120 days postinjection.
Ultrastructural evaluation was performed in randomly selected samples.
Immunofluorescence studies were negative, and ultrastructural evaluation was compatible with nephroangiosclerosis with secondary segmental/global glomerular sclerosis and confirmed the tubular damage.
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To study the effects of curcumin on MSCs after 14 days of cultivation in three-dimensional high-density culture, ultrastructural evaluations were performed.
The physical properties of both the C and C/Fe particulates were such that sections for ultrastructural evaluation could be prepared without sectioning artifacts.
The ultrastructural evaluation results are in agreement with the other parameter findings, which reveal that there is no significant deterioration of liver tissues in IONPs administered Wistar rats compared to iodine.
Evaluation was made of morphological and ultrastructural changes.
Evaluation was not speeded.
For ultrastructural evaluations sections were cut at a thickness of 70 80 nm, counterstained with uranyl acetate and lead citrate (Reichert Ultrastainer, Leica, Germany), and examined in a Zeiss EM 109 transmission electron microscope and a digital camera (2 K, Zeiss, Oberkochen, Germany).
For ultrastructural evaluation, resin blocks were sectioned in the transverse plane at 100 nm intervals, stained sequentially with uranyl acetate and lead citrate and examined with a Hitachi® 7500 transmission electron microscope at 100 kV.
Ultrastructural evaluation of the myelin was performed on myelinating cultures that had been harvested at 4 5 weeks in vitro.
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