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Selected vibratome sections, permeabilised with ethanol instead of Triton, were processed for the ultrastructural detection of aconitase using a standard immunoperoxidase procedure using the Vectastain ABC kit (Vector) and diaminobenzidine tetrahydrochloride (DAB) as a chromogen.
Some additional sections were used for the ultrastructural detection of CB1R CB2R heteromers, as indicated below.
These data were corroborated here following the ultrastructural detection of PLA-stained material for CB1R CB2R heteromers.
Ultrastructural detection of CB1R CB2R heteromers was carried out using the PLA technique followed by immunogold labeling and silver enhancement.
Fragments of the same samples were submitted to cytochemical staining using cuprolinic blue for the ultrastructural detection of PGs [ 29].
Ultrastructural detection of lanthanum nitrate applied into the lateral ventricle of a hyh mouse, at P20. (a-c ; c is a detailed view of the area framed in b ) Lanthanum penetrates from the ventricular lumen towards the brain parenchyma through the winding extracellular spaces of the astrocytic layer (as ) lining the denuded ventricular surface (black arrows ).
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For the nanoparticles to be applicable as labels for ultrastructural detection, they need to be coupled to biomolecules targeting them to the molecules of interest.
Previously, several groups have been used immunohistochemical or immunofluorescence detection for determine the localization of influenza virus antigens, as well as in situ hybridization for detection of viral sequences and ultrastructural examination to detect viral particles, in cases of fatal H1N1 influenza A virus infection during the period 2009 2010 [ 13- 19].
Inefficient photoconversion of DAB into polymers by GFP compared to other fluorophores hampers the broad application of more direct detection of GFP at the ultrastructural level.
Together with virus isolation from the skin, the detection of viral antigens and ultrastructural observation of the virions in the feather epidermis raise the possibility of feathers as sources of infection.
Immunocytochemical procedures for the detection of the sst2A receptor at the ultrastructural level were performed as previously described [30], [39].
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