Research is also ongoing in the fields of cerebrovascular physiology, cerebral ischemia, cellular and system neurophysiology, cellular and molecular neurobiology, neuroanatomy at the light and ultrastructural level, and imaging.
Scientific and medical illustration and imaging.
See-through medaka (STII strain) were exposed for 96 h using the biliary toxicant alpha-naphthylisothiocyanate (ANIT) as a reagent, and fish were evaluated daily using histological and ultrastructural analysis, and by imaging directly through the body wall of living fish.
SDHD was investigated on the genome, transcriptome and proteome level using mutation screening, methylation specific PCR, real-time quantitative PCR based homozygous deletion screening and mRNA expression profiling, immunoblotting, functional protein analysis and ultrastructural imaging of the mitochondria.
We developed a method to achieve systematic nanoscale molecule mapping in dense tissue sections using STORM and ultrastructural imaging on the same samples using EM.
In addition, since µMRI resolutions sit between those of ultrastructural optical/electronic imaging and clinical MRI, the µMRI projects provide an ideal intermediate-resolution platform to coarse-grain the ultrastructural results for an eventual clinical application.
Importantly, ultrastructural imaging and subsequent detailed lysosomal activity analyses revealed reduced basal autophagic degradation and the accumulation of defective mitochondria in DJ-1 KO cells, that was linked with decreased levels of phospho-activated ERK2.
The purified MG23 preparations were used for chemical cross-linking, ultrastructural imaging, and channel recording.
Glutaraldehyde-preserved samples were intended for ultrastructural analyses with TEM (see below) and also imaging with light microscopy; formalin-preserved samples were intended for quantification and/or imaging.
Quantitative aliquots of sediment samples were processed for metazoan enumeration and imaging, loriciferan searches, nematode morphotype identifications, and nematode and bryozoan ultrastructural analyses.
