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All thin sections (for immunogold labelling or ultrastructural analysis) were subsequently post-stained in 2% (w/v) aqueous 0.22 μm-filtered uranyl acetate for 1 h.
Apparent upon ultrastructural analysis were several lumen-like structures (canalis) formed by adjoining cells located in the inner regions of the MARY-X spheroid.
Blocks chosen for ultrastructural analysis were trimmed, sectioned at 60 nm, stained with lead citrate and uranyl acetate, and examined at 60 kV on a Philips 300 transmission electron microscope.
The expression of intermediate filament proteins and the chromosomal abnormalities were analyzed at passages 3 through 5 for both cell lines; the detailed study of the nestin cytoskeleton morphology using epifluorescence microscopy was carried out between passages 6 and 9. Confocal microscopy and ultrastructural analysis were performed using the GM7 cell line at passages 15 through 22.
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Ultrastructural analysis was performed by transmission electron microscopy.
Ultrastructural analysis was performed by transmission electron microscopy (TEM), and AgNPs were detected by TEM X-ray microanalysis.
The ultrastructural analysis was also performed on MCF-7 and MDA-MB231 cells exposed to BTB extract treatments.
Ultrastructural analysis was essentially as in Croce et al. [10].
Ultrastructural analysis was performed by TEM (Fig. 11).
To clarify the state of cell-cell contact at a high resolution, ultrastructural analysis was conducted.
As for the previous cohort, ultrastructural analysis was performed using electron microscopy and the number of myelinated axons per mm2 quantified.
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