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Ultrastructural analysis was performed by transmission electron microscopy (TEM), and AgNPs were detected by TEM X-ray microanalysis.
The ultrastructural analysis was also performed on MCF-7 and MDA-MB231 cells exposed to BTB extract treatments.
Ultrastructural analysis was essentially as in Croce et al. [10].
Ultrastructural analysis was performed by TEM (Fig. 11).
To clarify the state of cell-cell contact at a high resolution, ultrastructural analysis was conducted.
As for the previous cohort, ultrastructural analysis was performed using electron microscopy and the number of myelinated axons per mm2 quantified.
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Ultrastructural analysis is required to confirm unequivocally that labeling is located in the cytoplasm or membrane.
In situ ultrastructural analysis is suitable to study cellular metabolism because metabolic pathways have variable structural bases.
In vivo biodistribution studies as well as histological and ultrastructural analysis are in progress in our laboratory.
All thin sections (for immunogold labelling or ultrastructural analysis) were subsequently post-stained in 2% (w/v) aqueous 0.22 μm-filtered uranyl acetate for 1 h.
Apparent upon ultrastructural analysis were several lumen-like structures (canalis) formed by adjoining cells located in the inner regions of the MARY-X spheroid.
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