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Ultrastructural analysis on DTg muscles further confirmed that the sarcomeric structure was conserved in non-degenerative fibers (Fig. 4D asterisk).

To confirm the association of phosphorylated TDP-43 with the tau aggregates, we performed ultrastructural analysis on spinal cord from a JNPL3 mouse with robust neurofibrillary tau pathology.

The supernatant, containing smaller vesicles, was spotted on a carbon/formvar-coated electron microscopy grid and negative stained by 3% uranylacetate for ultrastructural analysis on a Tecnai 12 Biotwin transmission electron microscope at × 24 000 magnification.

Additionally, complementing the ultrastructural analysis on the glycocalyx, proteomic analysis determined that several glycocalyx-associated proteins, including versicans, aggrecans, proteoglycans, brevican, galectins, mucins, agrin, and clusterins were at least twofold downregulated in aged collectors as compared to adult ones (Fig. 3g,h and Table S1).

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A close relationship with the Apicomplexa was initially proposed based on the ultrastructural analysis of the zoospore, which revealed the presence of organelles resembling an apical complex [ 7].

Ultrastructural analysis of cells and particles was performed on random ultrathin sections of the cells on filter inserts using a CM12 transmission electron microscope (TEM, Phillips, Eindhoven, The Netherlands) operated at 80kVV.

Ultrastructural analysis of epoxy resin embedded hearts was performed on 70-nm sections.

Ultrastructural analysis of peripheral nerve of Pank2 −/− animals on a ketogenic diet showed the presence of swollen mitochondria, characterized by cristae degeneration and by the presence of amorphous material in the matrix (Fig. 5).

To our knowledge this report is the first ultrastructural analysis of the actions of the aqueous extract from P. angulata on Leishmania and could be helpful for understanding possible mechanisms of action of this agent on parasites and host cells.

A detailed ultrastructural analysis of the type species of Gyrodinium, G. spirale, was made based on cells collected from Skagerrak and southern Kattegat (Denmark).

Fig. 2 Ultrastructural analysis of plastids in the leaves of WT, fgl, and OPAO plants.

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