Her Majesty's Government Customs and Excise (UK) kindly donated confiscated ivory from illegal trade.
Second, the analyses of imports are based on UK Customs and Excise data, not all of which been validated by importing countries.
After ethidium bromide staining, reactive bands were visualized by a transluminator (Ultraviolet Light Box, GBox, SYNGENE, Synoptic Group, UK) and then were excised from the gel and cDNA was recovered by using EZ-10 Speed Column DNA kit (Bio Basic, Ontario, Canada).
"What we know is that intelligence is becoming central to anti-doping, and what the passport is, is one piece of intelligence that feeds into that; and linked to that as well are our relationships with UK border authorities, customs and excise and the police.
A SDS PAGE gel was transferred onto PVDF membrane (Invitrogen, UK) and the protein bands excised and analyzed via N-terminal sequencing (Department of Biochemistry, University of Cambridge).
Tumour samples were washed in Dulbecco's modified Eagle's medium (DMEM; BioWhittaker, Wokingham, UK) and macroscopic necrotic tissue was excised with a scalpel and washed in DMEM.
However, the transport secretary said UK taxpayers would not incur higher vehicle excise duty on their cars.
Tax disc, and its close relation Vehicle Excise Duty, were introduced in the UK in the Roads Act 1920.
Sub-clones were generated by re-transfecting an individual clone with a 2.334 kb SV40 promoter-hygromycin phosphotransferase cDNA fragment excised from pcDNA3.1 (hygro) plasmid (Invitrogen, UK) using PvuII (Promega, UK) and purified following agarose gel electrophoresis.
The hCx43 coding sequence was excised from pDest40.hCx43 plasmid (GlaxoSmithKline, Harlow, UK) using NdeI and AgeI restriction endonucleases and cloned into pRRLsincppt.CMV.polyAB.IRES2.EGFP.wpre construct via NdeI and AgeI sites generating pRRLsincppt.CMV.hCx43.IRES2.EGFP.wpre.
