Sentence examples for ug of tissue from inspiring English sources

Sixty (60) ug of tissue extracts were resolved on a 10% SDS-PAGE gel and analyzed for DEK expression by western blot using a monoclonal antibody (BD Bioscience, San Diego, CA).

PCR was performed using 1 ug of prostate tissue DNA in a 100 ul reaction volume using standard conditions of 94°C for 30 sec, 50°C for 30 sec, and 72°C for 45 sec for 40 cycles on an ABI 9700 thermalcycler (Foster City, CA).

40 ug of protein from tissue samples and 20 ug of protein from metabollically labelled TPC1 cells were mixed in triplicate before SDS-PAGE.

Taking into account the normalisation factors derived from the reference proteins, 10 ug of protein from tissue lysates of normal, FA, FTC and PTC samples were separated on 4-12% Bis-Tris NuPAGE gels and electroblotted onto nitrocellulose (Bio-Rad).

An amount of 2 ug of total RNA isolated from CRC tissues and corresponding normal tissues were reverse-transcribed into cDNA, and labeled with Cy3 or Cy5.

In fact, in an experimental model of crescentic glomerulonepritis, UG was able to attenuate renal inflammation by modulating the expression of tissue transglutaminase [ 22, 23].

However, initial studies required relatively large amounts of tumor tissue (typically 4 40 ug of total RNA).

300 ug of tumor, lung and bone whole tissue lysates were used and manufacturer recommended protocols were followed.

10 ug of total RNA from the indicated tissues was loaded on 20% acrylamide denaturing gels and transferred to Zeta-probe GT genomic blotting membranes (Bio-Rad) by electrophoresis.

Dried delipidated tissues were rehydrated by incubation for 2 hours in versene buffer followed by addition of 10 ug papain/mg dry tissue.

Reverse transcription of 1 ug of isolated total RNA from whole-lung tissue (same RNA as were used for the microarray part) was performed using the Tetro cDNA synthesis kit (Bioline) with random hexamer primers following the manufacturer's instructions.