Sentence examples for ug from each from inspiring English sources
Equal amounts of protein (500 ug) from each sample were subjected to immunoprecipitation (IP) with mouse anti-SUMO-1(GMP1, Invitrogen, Carlsbad, CA, USA) using nuclear complex co-IP kit (Active Motif, Carlsbad, CA), and then subjected to SDS-polyacrylamide gel electrophoresis.
Approximately 10 ug cDNA from each of the two flower samples were used for sequencing on a GS-FLX platform.
Approximately 10 ug cDNA from each of the four fruit flesh samples was used for sequencing on a Roche/454 GS-FLX Titanium instrument.
Protein concentrations were quantified using a Bradford assay (BioRad Protein Assay reagent), then 10 35 ug protein from each was loaded on 7.5% acrylamide gel then transferred to 0.45 um nitrocellulose membrane.
Total RNA (2 ug) from ovarian tissue was converted to cDNA using Superscript III (Invitrogen) primed with random hexamers under conditions described by the supplier.
For the identification of potential LARK targets, equal amounts of RNA (about 3 ug) from the total RNA sample or the immunoprecipitated LARK-RNPs were labeled and hybridized to Drosophila Genome Arrays chips.
For northern blot analyses total RNA (20 ug) from leaves was fractionated on 1.5% agarosa-MOPS 1× gel and blotted onto nylon membranes (Hybond-N+, GE Healthcare Life Sciences, Amersham, Argentina).
Total RNA (10 ug) from 46 embryonic kidney (15.5 dpc) from 5 litters of CD1 mice was put through one round of poly (A) selection (Oligotex Kit, Qiagen) followed by ribosomal depletion (Ribominus Kit, Invitrogen) to select mRNA.
Since the immuno-depletion column employed removes from approximately 65%to85%5% of the total plasma protein (Agilent, personal communication; [ 20]), the amount of protein loaded in the 09_OGE_6_depl_sample was correspondingly reduced to 650 ug to compensate for the loading of the non-depleted 1800 ug from 08_OGE.
Bisulfite modification was performed on 0.3 ug of DNA from each sample using the EZ DNA Methylation-Gold™ Kit (Zymo research), according to the instruction manual.
Around 6 8 ug of peptides from each fraction were analyzed using a nanoflow LC coupled to a QSTAR Pulsar i ESI-hybrid Q-TOF tandem mass spectrometer (Applied Biosystems/MDS Sciex).
