And robots will be ubiquitous -- driving us everywhere, homeschooling our children, and maybe even becoming preferred sexual partners.
The electroporation of living embryos has been extensively used in gain-of-function studies, for which the expression vectors carry a ubiquitous promoter driving the transgene transcription, as well as in loss-of-function approaches, such as those using siRNA or morpholinos (reviewed in [6]).
A gene can be placed under the control of a ubiquitous promoter (driving the expression of the gene in all cells at all times) or self promoter (driving the expression of the gene under the control of its normal promoter), but transcriptional STOP sequences, which are flanked by loxP sites, can be inserted to block its expression.
The cloud and more ubiquitous connectivity is driving a significant increase in the blending of informal and formal learning.
Serotype 5 AAV vectors containing either a photoreceptor-specific, rhodopsin kinase (hGRK1) or ubiquitous (smCBA) promoter driving expression of wild type murine GC1 were subretinally delivered to one eye of P14 GC1KO mice.
This vector includes a mouse H1 RNase promoter driving ubiquitous expression of shRNA and a cytomegalovirus-immediate early (CMV) promoter driving GFP and neomycin fusion expression.
This was despite elav c155 −Gal4 driving ubiquitous neuronal expression (Fig. 5B S52R, S52R, H338R and G392R panels, and enlarged details for S52R and G392E).
The BAC had a ubiquitous actin promoter driving green fluorescent protein (GFP) expression, resulting in stable transgenic fish that were both green and homozygous mutant at the endogenous tif1gamma locus.
To identify the postembryonic lineages generated by the four labial expressing neuroblasts, we performed a MARCM-based clonal analysis with an ubiquitous tub-Gal4 driving UAS-mCD8 GFP UAS-mCD8 GFP 1999; Lee and Luo, 2001999
To construct vectors driving ubiquitous transgene expression in ES cells and mice, the PCR amplified cDNAs were digested with EcoRI and inserted into the EcoRI sites of pCAGGS [ 18] to generate pCX-ECFP, pCX-EYFP and pCX-DsRed.
This adds evidence for the hypothesis that energy metabolism as a complex trait plays an ubiquitous role in driving the adaptive divergence between dwarf and normal whitefish while other candidate adaptive traits play more tissue and context specific roles.
